ddPCR to innovate replication-competent virus testing
The latest Droplet Digital™ PCR (ddPCR™) technology and kits could provide a time-saving
solution to transform replication-competent virus (RCV) testing in gene therapy workflows, helping to ensure
the safety of new therapeutics. For Dipika Gurnani, a Product Manager at Bio-Rad Laboratories, the promise of
these approaches is an everyday focus. “My passion has always centered around rare diseases that have lacked
effective diagnosis and treatment options,” she explains. “My primary objective at Bio-Rad is ensuring that
the products in our gene therapy portfolio reach end-users and help researchers to bring safe and efficacious
therapies to the market.”
A step-by-step workflow for a HPRT knock-out experiment in an adherent cell line
This step-by-step tutorial video demonstrates the process of conducting a HPRT
gene knock-out experiment in a HEK293 adherent cell line. See how to design the experiment; prepare both
cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; and isolate
and clonally expand the culture for further experimentation.
What next for CRISPR after first therapeutic approvals?
As we celebrate the first regulatory approval of a gene-editing based
therapeutic, Casgevy, for the treatment of sickle cell disease and transfusion-dependent β-thalassemia, we
consider the gene-editing challenges that must be overcome before CRISPR gene-editing technology becomes a
common treatment modality.
Explore the latest developments in cell and gene therapy with fellow scientists,
manufacturers, and regulatory experts.
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