Antibodies

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Western Blot: V5 Epitope Tag Antibody [NB600-381] - Analysis using the HRP conjugate of NB600-381. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

V5 Epitope Tag Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB600-381
Host: Rabbit
Reactivity: Not Species Specific, Escherichia coli
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blotting

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml

A20/TNFAIP3 Antibody (59A426)

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-77533
Host: Mouse
Reactivity: Human, Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blotting

Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.

GFP Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB600-303
Host: Rabbit
Reactivity: Not Species Specific, Human
Applications: Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting

Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - IHC-P analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong nuclear staining of PHD3 primarily in the cancer cells while the stromal cells were largely negative for this protein.

EGLN3/PHD3 Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-139
Host: Rabbit
Reactivity: Human, Mouse
Applications: Electron Microscopy, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting

Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

p19ARF Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB200-106
Host: Rabbit
Reactivity: Mouse
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

PGC1 alpha Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-04676
Host: Rabbit
Reactivity: Hamster, Human, Mouse, Squirrel
Applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunoprecipitation, Western Blotting

Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

Laminin Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB300-144
Host: Rabbit
Reactivity: Human, Mouse, Rabbit (Lapine), Sheep (Ovine)
Applications: Functional Studies, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting

Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.

RPE65 Antibody (401.8B11.3D9)

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-355
Host: Mouse
Reactivity: Bovine, Human, Mouse, Pig (Swine)
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting

Flow Cytometry: CD31/PECAM1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM1 (MEC 7.46) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).

CD31/PECAM-1 Antibody (MEC 7.46)

Manufacturer: Novus Biologicals, LLC
Catalog Number: NB100-1642
Host: Rat
Reactivity: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections)

Immunocytochemistry/Immunofluorescence: PCSK9 Antibody [NBP2-31364] - PCSK9 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.1 ug/ml was used. Image objective 40x.

Proprotein Convertase 9/PCSK9 Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP2-31364
Host: Rabbit
Applications: Immunocytochemistry