Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401814)" loading="lazy" width="300" height="225" decoding="async" data-nimg="1" style="color:transparent" sizes="(min-width: 630px) 190px, 30vw" srcset="https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=256&q=75&fit=clip&auto=format 256w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=384&q=75&fit=clip&auto=format 384w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=640&q=75&fit=clip&auto=format 640w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=750&q=75&fit=clip&auto=format 750w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=828&q=75&fit=clip&auto=format 828w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=1080&q=75&fit=clip&auto=format 1080w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=1200&q=75&fit=clip&auto=format 1200w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=1920&q=75&fit=clip&auto=format 1920w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=2048&q=75&fit=clip&auto=format 2048w, https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=3840&q=75&fit=clip&auto=format 3840w" src="https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/8349fbeada20d30f22162176146cc580cd7511ae-182x400.jpg?w=3840&q=75&fit=clip&auto=format">
SIX1 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-84264
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Analysis of HeLa, using vimentin antibody at 1:200 dilution.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/03952da303f5a4d8940ca669f2ec9079021f2d3d-227x400.jpg?w=3840&q=75&fit=clip&auto=format)
Vimentin Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-31327
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunoprecipitation, Western Blotting
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/c0261394b084604a8a97f756fc717a2f8772f57c-220x400.jpg?w=3840&q=75&fit=clip&auto=format)
PPAR alpha/NR1C1 Antibody (3B6/PPAR)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB300-537
- Host
- Mouse
- Reactivity
- Bovine, Human, Mouse, Rabbit (Lapine)
- Applications
- Flow Cytometry, Gel Super Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting
![Immunocytochemistry/Immunofluorescence: PDI Antibody [NB100-1921] - PDI staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4ae76bf8db43715d9a9aae46b22c87235da12369-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
PDI Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-1921
- Host
- Rabbit
- Reactivity
- Bovine, Human, Mouse, Primate
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunoprecipitation, Western Blotting
![Immunohistochemistry-Paraffin: TNF-alpha Antibody [NB600-587] - Paraffin-embedded sections of human artery tissue sections.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/71239d355ba40f7e33d21d5632c9495297d70830-400x284.jpg?w=3840&q=75&fit=clip&auto=format)
TNF-alpha Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB600-587
- Host
- Rabbit
- Reactivity
- Human, Mouse, Primate
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Immunocytochemistry/Immunofluorescence: Adiponectin Antibody [NB100-65810] - Immunocytochemical staining of HL60 cells with Rabbit anti Human adiponectin.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/cc3b90bfb070175abc5c345ea1de877b73a49b7e-400x391.jpg?w=3840&q=75&fit=clip&auto=format)
Adiponectin/Acrp30 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-65810
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blotting
![Immunohistochemistry-Paraffin: Bid Antibody [NB100-56106] - Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/82ce6e4945361cf9e2b3c189f8b2b6a6304ddaef-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
BID Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-56106
- Host
- Rabbit
- Reactivity
- Dog (Canine), Gerbil, Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Immunohistochemistry: BMP-2 Antibody [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/9a06ab285c6ee035c3514597783a74ed54b60683-334x400.jpg?w=3840&q=75&fit=clip&auto=format)
BMP-2 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-19751
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Flow Cytometry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/16efeb29de20bcb1ccc081e88c77dbbaf6b0fbd7-400x188.jpg?w=3840&q=75&fit=clip&auto=format)
Desmoglein-3 Antibody (5G11)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-1643
- Host
- Mouse
- Reactivity
- Human
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a00cee6f342bdc15e374de57789bb305826f39e9-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
HDAC3 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-1669
- Host
- Rabbit
- Reactivity
- Human
- Applications
- Chromatin Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ba808ffb2676ff55a65a4f07a21ae29e1576f63b-400x368.jpg?w=3840&q=75&fit=clip&auto=format)
HDAC5 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP2-22152
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Flow Cytometry: NG2 Antibody (LHM 2) [NB100-2688] - NG2 antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4197244a9834f2d014cab677a580351c11f203ab-400x354.jpg?w=3840&q=75&fit=clip&auto=format)
NG2/MCSP Antibody (LHM 2)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB100-2688
- Host
- Mouse
- Reactivity
- Human
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Immunocytochemistry/Immunofluorescence: OLFM4 Antibody [NBP2-24535] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OLFM4 (NBP2-24535) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0732f31d274ae6665576d0e3f3a22e668dd607e7-400x313.jpg?w=3840&q=75&fit=clip&auto=format)
OLFM4 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP2-24535
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- Immunocytochemistry, Immunohistochemistry, Western Blotting
![OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/18bdbc4d1d62eeaf27410b9da638abf8303c6ea5-400x327.jpg?w=3840&q=75&fit=clip&auto=format)
OLLAS Epitope Tag Antibody (L2)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-06713
- Host
- Rat
- Reactivity
- Not Species Specific
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Western Blotting
![Western Blot: POT1 Antibody [NB500-176] - Detection of POT1 in HeLa nuclear extracts (25 ug) using NB500-176(1:500). ECL detection 1 min.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5473754e6d8241e9b49106cadbb7b71b36315295-229x400.jpg?w=3840&q=75&fit=clip&auto=format)
POT1 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB500-176
- Host
- Rabbit
- Reactivity
- Human
- Applications
- Immunocytochemistry, Western Blotting
![Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/76a8c719ac17fe69a33ab511f8f3131dd0de9da7-400x298.jpg?w=3840&q=75&fit=clip&auto=format)
VCAM-1/CD106 Antibody (6G9)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-47491
- Host
- Mouse
- Reactivity
- Human
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Western Blotting
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/33072b993d0d3e38a1d21b3e15652d782762925c-159x400.jpg?w=3840&q=75&fit=clip&auto=format)
Clathrin Heavy Chain 1/CHC17 Antibody (X22)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB300-613
- Host
- Mouse
- Reactivity
- Bovine, Hamster, Human, Mouse
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunomicroscopy, Immunoprecipitation, Western Blotting
![Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of HeLa Cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/18fafe2279cf39d8ce49712e0a709dc9133a299e-158x400.jpg?w=3840&q=75&fit=clip&auto=format)
pan Actin Antibody (ACTN05 (C4))
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB600-535
- Host
- Mouse
- Reactivity
- Bovine, Human, Mouse, Pig (Swine)
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting