![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e7d987fde2183d50001afc15c20c088b2ff4c470-400x353.jpg?w=3840&q=75&fit=clip&auto=format)
ROBO1 Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB600-1254
- Host
- Rabbit
- Reactivity
- Dog (Canine), Human, Mouse
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Flow Cytometry: RUNX3 Antibody (2B10E8) [NBP2-22391] - Intracellular flow analysis of human lymphocytes probed with Runx3 antibody (red) and isotype control (green) at 0.25 ug/10^6 cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b8571c418a42e1ad57c01bca1054d055d344ea3d-400x383.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: S100 calcium binding protein A14 Antibody [NBP1-90000] - Staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (microtubules).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a3d1713d5efa5f90cb1e8b28b56f880b65b67dc8-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: S100A6 Antibody [NB110-93274] - Detection of S100A6 antibody in Hela whole cell extracts using NB110-93274.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7f5bd6f343099bbf26561c3690f97766ca3f5942-245x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: SARM1 Antibody [NBP2-29625] - SARM1 antibody was tested in SHSY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6a1e2602f4dcb96f9cd63722902db312c5a414a8-400x303.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SLC26A7 Antibody (14H5) [NB110-60965] - Detection of Slc26a7 using NB110-60965. Tx: Okp cells transiently transfected with mouse Slc26a7 UTX: untransfected Okp cells.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/a88faf9d11cb5f505b90789e777b217cc171a1c7-315x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: SLC34A2 Antibody [NBP1-81013] - Human lung.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/31ed7dde4da28daf14496dfcbc653f7a5ffe9eb8-389x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SLC46A3 Antibody [NBP1-85054] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/319ab8e50e0f76c54d8b1860b61ca4ae2d716fd7-328x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: BAG4 Antibody [NB100-56696] - Analysis of 30 ug of total cell lysate from HeLa cells with anti-SODD at 2 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/00436b39c1cb6d1b006f900e2d01c4cfff8cf3c6-277x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Somatostatin Receptor 5 Antibody [NB100-56498] - analysis of human placenta using Somatostatin Receptor 5 antibody at 10 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/78f3aeb0ea7f73b9ec8c87a936cec8813d18946c-400x317.jpg?w=3840&q=75&fit=clip&auto=format)