![Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Human laryngeal squamous cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/25a847c7ad4747f9ab867bcfc3ba7a7be75d833f-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
ASH2L Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB600-250
- Host
- Rabbit
- Reactivity
- Human
- Applications
- Chromatin Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting, Proximity Ligation Assay
![Proximity Ligation Assay: Claspin Antibody [NB100-247] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human Claspin (NB100-247). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1a7b37fecd1fed43140405718f9073d22a647cda-400x303.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: GW182 Antibody [NBP1-28751] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28702, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ce10ea5e527113e6b3c36cf649a779faa6451354-400x264.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Musashi 1 Antibody [NB100-1759] - Musashi-positive cells stained with Musashi antibody (1:150 dilution) in the ventricular zone of E14.5 mouse medulla. Image taken at 200x magnification. The sections were 4% PFA fixed, paraffin-embedded and cut at 5 microns.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f9c7751be2a501e3a0a3871ac8cc45854e579709-400x323.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Rhot1 Antibody [NBP1-59021] - Fetal Lung lysates, Antibody Dilution: 1.0 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6dde74276ae070b447751d4e9ec553de2acfd0e1-400x267.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b7d5843e68f8cfa39dc783326eee94dcb1d782ff-400x304.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: SC35 Antibody (SC-35) [NB100-1774] - HS-68 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-Splicing Factor Clone: SC-35 diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, FITC-conjugate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/12ba684bd6163a6bbb525bb7a92473cf514b755b-400x321.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: metallothionein Antibody (UC1MT) [NBP1-97493] - Human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4f6ee94c010c1cec498f5a46b54ad85d98296f97-400x316.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/21cbc1f6cc5b305cbedc5c2284e19b2721c2f377-400x304.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7ab18e1a5bb67c9006b1d2ebd0e72c7bbee8bddc-400x302.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b70e64797e3caf6cde4b7bb6a2ff8d1d78a710a9-400x391.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Caspase 14 Antibody [NB100-56126] - Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for Caspase-14 expression using this antibody at 1:2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1). Decreased Caspase-14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0c8c6f76e7a7dc481a145744c956d201f9182b3a-400x274.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Chd7 Antibody [NBP1-77393] - Antibody was tested in NIH/3T3 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ceb9d83c9e2eb6472c1f84ffdf55ffc75b0109ad-400x297.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d94595cf5853f3b48f8ce0e51b061061e08267c3-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/af0834282430b53dcf06f5416cdd92164da83ae2-189x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Collagen VI Antibody [NB120-6588] - Analysis using the Biotin conjugate of NB120-6588. collagen VI antibody 1:400 45 min RT showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/3d244c5a6b9f572d119aee52c19a0f45068a3a06-400x154.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0515e68c8f9ee8cec0f27dd3434c4d969ebde8f3-195x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: EZH1 Antibody [NB100-56358] - Detection of EZH1 in mouse (lane 1) and human (lane 2) spleen lysate using this antibody at 2 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7082997c65bbef9d2f82ff2c1aa833332983a660-221x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Lin28 Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysate](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e73dfcf73135ad762c323ee1fa67c1360644e1c5-190x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: p40 (p63 delta) Antibody [NBP2-29467] - Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with p40 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note nuclear staining.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4578bc9c73a6bf97f8941c70362c2f3b7a7c3070-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: RTEL1 Antibody [NBP2-22360] - RTEL1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5d83e655389cbf60356043350686638b3648b14b-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Snail Antibody [NBP2-27184] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with SNAIL (NBP2-27184) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red). Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/c620224f4ec9c9dedab3827d57e7222b74edbe6a-400x302.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SOX17 Antibody [NBP2-24568] - analysis of SOX17 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using SOX17 antibody at 1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/be3c58bee5760a7d78f99193449d16fffb3aa87c-334x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: CVID Antibody [NB100-56516] - Western blot analysis of A) human spleen, B) mouse spleen, C) human stomach, D) and mouse stomach lysate using TACI antibody at 2 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/28951e9f60654f75f04510c1753a490d443fb141-233x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fab6bf9294aa6191fd41515f0ae39e2fb62c5a40-400x296.jpg?w=3840&q=75&fit=clip&auto=format)