![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e0c5df51098ba4a48e554bb6c0a4cf83ed086c23-400x302.jpg?w=3840&q=75&fit=clip&auto=format)
PGC1 alpha Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-04676
- Host
- Rabbit
- Reactivity
- Hamster, Human, Mouse, Squirrel
- Applications
- Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Frozen Sections), Immunoprecipitation, Western Blotting
![Immunocytochemistry/Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/780f5e3c30cb59839888f63e2a931c62d309ab2e-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ad9151bf265a37ca610fad5354a659dff4bef9dd-400x299.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: CD31/PECAM1 Antibody (MEC 7.46) [NB100-1642] - CD31/PECAM1 (MEC 7.46) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1869cf622f231a8b48921f542b87bd2e61df3d8b-400x386.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PCSK9 Antibody [NBP2-31364] - PCSK9 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.1 ug/ml was used. Image objective 40x.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4f2105aaf9c49f2033002fae7b2b556f5d664342-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Delta Np73 Antibody (38C674.2) [NBP2-24873] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Delta Np73 (38C674.2) NBP2-24873 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d2f06811698ecc8c5397337d799c07b8847e3112-400x326.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry: GAPDH Antibody [NB300-320] - Staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/e6b3bdad4e4f5f51d86792d5eb0462cad087dc54-400x387.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [NB100-303] - analysis of PHD3 in ARPE-19 cells using anti-PHD3 antibody. Image from verified customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bd3ac318ad57a267f4759ea114e502064ee2b281-400x299.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: Ki67 Antibody [NB110-89717] - Staining of mouse bone marrow cells using NB110-89717 at a dilution of 1:100. Photo courtesy of product review by verified customer.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/fbaf2e7e267f7d5c2b51148a90be60da239d6d02-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: IkB-alpha Antibody (6A920) [NB100-56507] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6cfd5588b2dfe02fc17cb10d27b83ff777fcc4cc-400x351.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7e886cb8786e78d72e9fe26a7c7036039aac5d80-400x314.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image courtesy of anonymous customer review.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/6d9ab76f12a0f6974f05a54deb09d69549082845-400x305.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - U2OS cells](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b03c4ca0bfe50ddb63adbb5a061007d8b43498d4-400x332.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis of TLR4 using NB100-56723 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bc0161246a3ee649d91b1a1482bb9bead9566367-400x368.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/168014d4c767ae07f871352548b9b3d8caa87f25-400x317.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody [NB100-110] - Staining of skin epidermis](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5a15c0b048f0c3d24ee231a4dd0accb827c9b605-400x315.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control (Sigma, Cat. No. M5409); purple represents anti-TLR2 antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4497eed3abc787f0cd1ec32227bc72443326bc96-400x329.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the FITC conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with L243 FITC.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bf7b44c66ee7e91b8ec25172a673e4dcbacb8c22-370x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (JC/70A) [NB600-562] - Analysis using the Biotin conjugate of NB600-562. Comparison between mouse (lung, top) and human (prostate, bottom) staining using anti-CD31 antibody. Data demonstrates that the antibody stains human, but not mouse CD31. Image from verified customer revie](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ee6e6df8a8cbb7f33cc6d925069325e0a75e6e9c-400x376.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-48320] - Immunocytochemical analysis of SQSTM1/p62 in HeLa cells](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/be6916110aaa144582869ba03be68626f804f221-400x296.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NB200-157] - Human colon carcinoma.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0f39c873d71b8728762fe3a2b353e2109d7412da-352x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f0c646b30bab4aa3ce5dea4f4561cf3de83530a3-242x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SCP3 Antibody [NB300-232] - Analysis of SCP3 in mouse testis protein.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7445fc32359f9a27fd8401940dbcc239430c4a5d-211x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow (Intracellular): TLR8 Antibody (44C143) [NBP2-24917] - Analysis using the PE conjugate of NBP2-24917. Staining of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR8 antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/82e5e067c902a55f971420f213a33bccddd8d234-400x309.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: IKK alpha Antibody (14A231) [Azide Free] [NBP2-27409] - Intracellular staining of HEK293 cells using 0.5 ug of IKKa antibody (red) and isotype control (green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibody.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/38177e6e1316546623a91021dfc51976d64eaf8a-400x340.jpg?w=3840&q=75&fit=clip&auto=format)