![Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/0e506200370ff87b9b3dd5680d8e84fd825c4cee-400x300.jpg?w=3840&q=75&fit=clip&auto=format)
HAP1 Antibody (1B6)
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NB110-74569
- Host
- Mouse
- Reactivity
- Human, Mouse
- Applications
- Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Immunoprecipitation, Western Blotting
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4414554a7b2ef1952c8d5317bb170de8bb9b57cd-371x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 [ac Lys4] Antibody [NB21-1024] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/24e849cde63602dcbc1dabb4908f24a4dc08fba0-400x291.jpg?w=3840&q=75&fit=clip&auto=format)
![Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Dot blot analysis of HIstone H3pS10 with picomoles of peptide.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/f85d8ec9bc4155d739c316f7405894aafcbfb1aa-400x370.jpg?w=3840&q=75&fit=clip&auto=format)
![Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE. Total viable cells were used for analysis.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1b29d941219a99c58ab1b25d949503567f7bd624-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/caa329186f66f925d1f8051880d95e0fe25f22a0-170x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2f0970f9f85cdce8f0d2d267e14f313566cb15f3-400x286.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in transfected cells (0.1 ug) and Hey1B (100 ug)](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/68d8b76f48334c730a0bb92d4ba3d29b2668c686-342x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Angiotensinogen antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4f943eb6019ca9bdce90f9c04eb4cb8e01af7a28-400x296.jpg?w=3840&q=75&fit=clip&auto=format)