Antibodies

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Western Blot: ARHGAP22 [p Ser22] Antibody [NBP1-44072] - ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either a null vector (CTRL), ARHGAP22, ARHGAP22-serine 22 to alanine mutation, ARHGAP22 serine 397 to alanine mutation were electroblotted. The left panel was probed with the NBP1-44072 antibody, while the right panel was probed with the NBP1-44073 antibody, each at 1ug/ml. Each antibody recognizes the unmodified ARHGAP22. NBP1-44072 antibody recognized the S397-A mutation but not the S22-A mutation, while NBP1-44073 antibody recognizes the pS22-A mutation and not the pS397-A mutation. This data confirms the specificity of each ARHGAP22 phospho specific antibody.

ARHGAP22 [p Ser22] Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-44072
Host: Rabbit
Reactivity: Human, Mouse
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blotting

Immunohistochemistry-Paraffin: ERAS Antibody [NBP2-23727] - analysis of ERAS in paraffin-embedded formalin-fixed rat brain tissue using an isotype control (top, left) and this antibody (bottom left, and right) at 1:100.

ERAS Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP2-23727
Host: Rabbit
Reactivity: Human, Rat
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin-Embedded Sections)

Immunohistochemistry: Glucose Oxidase Antibody [NBP1-70120] - 10 ug/ml to evaluate staining on several mouseTissues. Moderate to strong staining was seen on manyTissues, with low background staining. This image shows Gli-2 staining of mouse testis.Tissue was formalin-fixed and paraffin embedded.

Glucose Oxidase Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-70120
Host: Sheep
Applications: Immunocytochemistry

IKK epsilon/IKBKE [p Thr501] Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-77829
Host: Rabbit
Reactivity: Human
Applications: ELISA, Immunocytochemistry, Western Blotting

Immunocytochemistry/Immunofluorescence: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390] - man HeLa cells were stained with monoclonal antibody NBP1-05390, which binds to a nuclear pore complex antigen, and chicken antibody to vimentin NBP1-05425.

pan Nuclear Pore Complex Marker Antibody (39C7)

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-05390
Host: Mouse
Reactivity: Human, Mouse
Applications: Immunocytochemistry

Western Blot: PIP5K2B Antibody [NBP1-44261] - Mouse brain homogenate samples were loaded on to a 10% Prep-gel Tris-Glycine polyacrylamide gels and blotted onto PVDF membranes. The membranes were probed with 0.2ug/ml of affinity purified NBP1-44261 antibody in 5% non-fat dry milk TBST over night at 4 C. The membrane was then further probed with goat anti rabbit HRP at 1:40, 000 and developed using a standard chemiluminescence method. A single major band at 54 kDa is observed

PIP5K2B Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-44261
Host: Rabbit
Reactivity: Chicken, Human, Mouse, Primate
Applications: ELISA, Immunocytochemistry, Western Blotting

Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.

PLK1 [p Thr210] Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-78021
Host: Rabbit
Reactivity: Human
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting

Immunohistochemistry-Paraffin: TNFAIP2 Antibody [NBP2-27085] - Analysis of human kidney probed with TNFAIP2 antibody at 5 ug/ml.

TNFAIP2 Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP2-27085
Host: Rabbit
Reactivity: Human, Primate
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin-Embedded Sections)

Immunohistochemistry-Paraffin: UNC5CL Antibody [NBP2-24576] - Formalin-fixed, paraffin-embedded human kidney stained with UNC5CL antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

UNC5CL Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP2-24576
Host: Rabbit
Reactivity: Human, Primate
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin-Embedded Sections)

Immunocytochemistry/Immunofluorescence: Cdk6 [p Tyr24] Antibody [NBP1-04958] - Staining of methanol-fixed Hela cells using CDK6 (phospho-Tyr24) antibody

Cdk6 [p Tyr24] Antibody

Manufacturer: Novus Biologicals, LLC
Catalog Number: NBP1-04958
Host: Rabbit
Reactivity: Human, Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting