Chromatrap®: a Fast, Reliable High Throughput ChIP-Seq Assay for Genome-Wide Protein-DNA Analysis

This application note demonstrates the utility of the Chromatrap® ChIP-seq kit to elucidate the genome-wide binding patterns of nuclear receptor binding events in human adenocarcinoma cells; highlighting the fast multiplex capability alongside massively parallel NGS. Mapping the chromosomal locations of TFs, nucleosomes, histone modifications, chromatin remodeling enzymes, chaperones, and polymerases is a key task in modern biology. The standard methodology for mapping these regulatory protein/DNA interactions is ChIP followed by massively parallel sequencing. Chromatrap® ChIP-seq assays now enable unbiased, high resolution, low noise amplification for genome-wide understanding of enriched protein-DNA regulatory networks.