Sample Preparation in Microbiome Analysis

Next-generation sequencing (NGS) methods for marker gene studies, metagenomics, and metatranscriptomics survey microbial communities with a different focus and detail. Standardization of the NGS workflow from sample collection, preparation, and storage is important in order to ensure that microbiome analysis is reproducible. In this application note, Eppendorf outline semi-automated liquid handling-supported workflows for evaluating diverse microbial communities.