Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo™ FX Accessory

Three dimensional (3D) spheroidal cell models have become a mainstay in life science research due to the ability to mimic in vivo-like environments. Performing media exchanges and washes with spheroids in cell repellent microplates can be problematic due to the risk of accidental spheroid removal. By incorporating a novel peristaltic pump-based tool, these procedures can be carried out in a controlled manner that eliminates spheroid disruption and removal, enabling long-term 3D experimental procedures requiring multiple media exchanges.