Rapid Development of a High Titer Protein Production Method with CHO Cells Transiently Transfected using MaxCyte Electroporation Technology

In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. Optimised protein expression protocols provide the ability to load cells with greater quantities of DNA relative to the standard CHO protocol. As a result, average protein expression per cell is increased. In addition, the effects of cell culture conditions on protein production in transiently transfected cells are illustrated. Finally, titers of soluble TNFαR are shown to increase for at least one week and are sustained for up to two weeks following electroporation of CHO cells with a sTNFαR expression plasmid.