The Thermo Scientific Nunclon Sphera Surface Supports Formation of Embryoid Bodies from Pluripotent Stem Cells

In this application note, the Thermo Scientific Nunclon Sphera is used for the in vitro formation of stem cell spheroids. The low binding property of Nunclon Sphera surface was demonstrated by low cell attachment of adherent cell lines as well as by the formation of embryoid bodies (EB) of both human embryonic stem cells and mouse embryonic stem cells. EB formation in Nunclon Sphera dishes was compared to either non-treated or cell culture treated dishes. These results demonstrate the advantage of the Nunclon Sphera surface for embryoid body formation in the process of pluripotent stem cell differentiation.