AbD Serotec – Your first choice for quality epitope tag antibodies

AbD Serotec, a division of MorphoSys, is a leading worldwide manufacturer and supplier of high quality antibodies and immunological reagents for scientific research. AbD Serotec offers a wide range of cell biology reagents including its hugely popular epitope tag antibodies, which are available at competitive prices and in bulk quantities.

What gives AbD Serotec’s epitope tag antibodies distinction is that they are:

• Manufactured to ISO 9001:2000 quality,
• Guaranteed to perform to specification,
• Suitable for a wide range of applications,
• Available directly conjugated to fluorochromes or enzymes of your choice,
• Supported by excellent technical services,
• And until December 31, 2006, they form part of our “Buy 4, pay for 3” promotion – see our website for full details.

Why not take a look at our complete range below and find out more using the “Request info” link?

V5 or Pk-Tag (clone SV5-Pk1)
V5 tags can be incorporated into expression vectors of bacteria, fungi/yeast, insect or mammalian origin. Its presence does not interfere with the transport and selection of recombinant proteins in bacteria or mammalian cells. Both transmembrane and secreted proteins have been tagged with V5. V5 is usually a 14 aa tag; GKPIPNPLLGLDST; although a smaller 9 aa epitope tag (underlined in the above sequence) has also been successfully used. Additionally, the 9 aa tag may be used as a peptide to inhibit the binding of the antibody to its native protein.

His or His-6 Tag (clone AD1.1.10)
His tag (HHHHHH sequence) is widely used for purifying proteins expressed in bacteria, yeast, insect and mammalian systems. In Western blotting of bacterial extracts, our antibody MCA1396GA has been shown not to cross-react with any endogenous products.

c-myc Tag
This is a popular expression vector tag of 10 aa (EQKLISEEDL). c-myc tag is often used by researchers using two colour immunofluorescence applications. AbD Serotec has 2 monoclonal antibodies, clone 9E10 and JAC6, for use in many applications.

GFP (Green Fluorescent Protein)
GFP is a naturally occurring 27 kDa protein derived from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. It is particularly useful in fluorescence microscopy techniques. AbD Serotec has three anti-GFP antibodies. The polyclonal antibody (AHP975) detects wild type, recombinant and enhanced forms of GFP and is reactive in both prokaryotic and eukaryotic expression systems. The two monoclonal antibodies (OBT1643 and OBT1820) are suitable for use in a variety of applications.

S-Tag (clone SBSTAGb)
The S-tag is a 15 aa (KETAAAKFERQHMDS) truncated form of the S-peptide, used in variety of protein expression applications in mammalian cells. The antibody is suitable for detection of S-Tag by flow cytometry and ELISA.

Strep-Tag (clone Strep-MAB and Strep-Tag II)
Strep-tags are short 8 aa sequences (WSHPQFEK), especially useful for tagging membrane proteins, metalloproteins and sensitive protein complexes with multiple subunits. Clone Strep-MAB (MCA2488) is particularly suited to the capture of Strep-Tag II containing proteins in assays such as ELISA or BiaCore. Clone Strep-Tag II (MCA2489/2489P) recognizes both the N- and C-termini, as well as internal Strep-Tag II, and is ideal for Western blot applications.

Glutathione S-transferase Tag (Clone vpg66)
Glutathione S-transferase (GST) fusion vectors are often chosen for mammalian protein expression studies because they are inducible, and give high-level intracellular expression of genes or gene fragments.

Maltose Binding Protein Tag (Clone R29)
Maltose Binding Protein is often used in protein expression studies because it creates a stable fusion product. This allows for easy purification from bacterial extracts under mild conditions.