amaxa's New Nucleofector® 96-well Shuttle™ System for High Throughput Transfection at Analytica

11 Apr 2006

Today, efficient and reproducible high throughput transfection remains a challenge. Using amaxa’s 96-well Shuttle, a high throughput extension for the well established Nucleofector platform, transfection of difficult-to-transfect cell lines and primary cells is no longer out of reach.

The 96-well Shuttle is a high throughput transfection system based on amaxa’s well established Nucleofector technology. Designed for applications such as RNAi based target identification and validation as well as reporter gene assays, it combines high-throughput capabilities with Nucleofector technology benefits.

This highly efficient, non-viral gene transfer method, suitable for primary cells (e.g. T cells) and hard-to-transfect cell lines (e.g. HL-60) is based on electroporation and uses a combination of optimized electrical parameters and cell-type specific solutions.

The Nucleofector 96-well Shuttle System is suited for the high throughput transfection of DNA, siRNA duplexes, and shRNA using the same transfection protocol. Transfection efficiencies of up to 95% with DNA and up to 99% with siRNA duplexes are achieved.

The system uses disposable modular standard 96-well Nucleocuvette plates. Each well can be addressed individually, i.e. 96 different transfection conditions can be tested in one plate, truly accelerating the optimization process.

By extending its sophisticated Nucleofector technology, amaxa sets a new standard for high throughput transfection, addressing both industrial and academic researchers.

Learn more about 96-well Shuttle applications at the Analytica in Munich and visit amaxa at booth #211 Hall A3 or listen to the presentation by Prof. M. Kiebler “High Throughput Target Validation in Primary Rat Neurons” on Thursday, April 29th at 11:30 a.m. in Room B21.

Links

Tags