AnaSpec Introduces New SensoLyte® Anti-Human MOG (1-125) IgG ELISA Kit

AnaSpec has announced the release of the newest addition to its Anti-MOG ELISA assay kit series – the SensoLyte® Anti-Human MOG (1-125) IgG Quantitation ELISA Kit. This kit, along with previously featured SensoLyte® Anti-Mouse MOG (1-125) and SensoLyte® Anti-Rat MOG (1-125) IgG ELISA assay kits are useful for determining the amount of anti-human MOG (1-125), anti-mouse MOG (1-125) and anti-rat MOG (1-125) antibodies, respectively. These kits, as well as the recombinant MOG (1-125) proteins are examples of innovative products from AnaSpec.

MOG (1-125) is found in the extracellular domain of Myelin Oligodendrocyte Glycoprotein (MOG). MOG is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it is demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys.1-6

SensoLyte® Anti-Human MOG (1-125)
Specificity : Human
Format : Human rMOG (1-125) precoated 12-strip plate
Standard(s) Included : Mouse & Rat anti-human MOG (1-125) IgG standards
Sensitivity : Detects as low as 1 ng anti-human MOG (1-125)
Assay Time : 2-3 hours assay time at room temperature
Sample Concentration : 0.5-1 μl of serum or cerebrospinal fluid
Read-out : Colorimetric read-out at 450 nm absorbance
Broad Dynamic Range : 8-500 ng antibody/ml serum (depending on colorimetric developing time)

Assay SensoLyte® Anti-Mouse MOG (1-125) Assay
Specificity : Mouse
Format : Mouse rMOG (1-125) precoated 12-strip plate
Standard(s) Included : Anti-mouse MOG (1-125) IgG standard
Sensitivity : Detects as low as 1 ng anti-mouse MOG (1-125)
Assay Time : 2-3 hours assay time at room temperature
Sample Concentration : 0.5-1 μl of serum or cerebrospinal fluid
Read-out : Colorimetric read-out at 450 nm absorbance
Broad Dynamic Range : 8-500 ng antibody/ml serum (depending on colorimetric developing time)

SensoLyte® Anti-Rat MOG (1-125) Assay
Specificity : Rat
Format : Rat rMOG (1-125) precoated 12-strip plate
Standard(s) Included : Anti-rat MOG (1-125) IgG standard
Sensitivity : Detects as low as 1 ng anti-rat MOG (1-125)
Assay Time : 2-3 hours assay time at room temperature
Sample Concentration : 0.5-1 μl of serum or cerebrospinal fluid
Read-out : Colorimetric read-out at 450 nm absorbance
Broad Dynamic Range : 8-500 ng antibody/ml serum (depending on colorimetric developing time)

References:
1. Linares, D. et.al. Protein Exp. Purif. 34, 249 (2004).
2. Bettadapura, J. et.al. J. Neurochem. 70, 1593 (1998).
3. Oliver, AR. et al J. Immunol. 171, 462 (2003).
4. Von Budingen, H. et.al. J. Clin. Immunol. 21, 155 (2001).
5. Lyons, J. et.al. Eur. J. Immunol. 29, 3432 (1999).
6. Von Budingen, H. et.al. Eur. J. Immunol. 34, 2072 (2004).