Automate western blotting with the BlotCycler Mini

Precision Biosystems have introduced a smaller version of the ingenious BlotCycler™ Multi, the BlotCycler™ Mini, a hands-free western blot processing system. With its simple, elegant design, BlotCycler™ Mini performs blocking, primary and secondary antibody binding, and washing steps in a rigidly precise fashion.

Reproducible assay results are delivered repeatedly because fluid distribution, timing, and shaking are automated, removing errors and variations that are proven to occur with manual blot processingⁱ . BlotCycler™ Mini, by Precision Biosystems, provides an affordable solution for labs intent on saving time and improving western blot quality and reproducibility.

“Any researcher interested in getting reproducible western blot assay results has to stop processing blots manually,” said Russ Yukhananov, Ph.D., CEO of Precision Biosystems. “Because BlotCycler Mini has a lower cost than our original BlotCycler, it offers an attractive solution for many labs,” he added.

Washing, blocking and antibody incubation are each an important step in western blot assays; if buffers, blocking agents and antibody are inconsistently applied, or the timing of any protocol step even slightly inaccurate, variations occur in assay results. With BlotCycler™ Mini, reagents and antibody are added to the system’s tanks, and blots are placed in the trays. BlotCycler™ Mini is equipped with 2 trays and can process from 1 to 8 blots with two different primary and secondary antibodies simultaneously. The operator uses the touchscreen to program blocking time (from 10 minutes to 120 hours), washing steps, the addition of antibodies, and incubation time. Each step of the protocol is performed automatically with robotic precision – adding and draining reagents, agitating membranes, adding the antibodies, and washing the trays. BlotCycler™ Mini works with new and established protocols, uses standard reagents, requires about 17.25” by 10.25” of bench space and works with standard electrical power.

ⁱ(Koller and Wätzig, 2005, Precision and variance components in quantitative gel electrophoresis, Electrophoresis, 26 (12):2470-24

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