Automating ForenSeq™ DNA Signature Prep Kit for Forensic Genomics Applications with ID NIMBUS^®

Hamilton Robotics have introduced the ID NIMBUS® assay ready workstation, specifically pre-configured and qualified to automate post-PCR sample processing using the well-known ForenSeq DNA Signature Prep Kit from Verogen. Forensic laboratories of any size, and especially those considering the implementation of NGS technologies into forensic casework, can now efficiently prepare NGS libraries from up to 96 DNA samples extracted from even the most challenging forensic evidence while maintaining a high degree of sample integrity for confidence in results. In addition to increasing throughput, the automated system also avoids repetitive strain and fatigue for users, and frees analysts to refocus their time on data interpretation and other activities.

The ForenSeq DNA Signature Prep Kit is part of a complete, fully validated DNA-to-data solution. This highly sensitive and accurate kit generates critical data that might be missed in conventional capillary electrophoresis analyses due to highly degraded DNA, low quality DNA or complex DNA mixtures. “Bringing increased efficiency to forensic laboratories is an honor for Verogen,” commented Dr. Cydne Holt, General Manager and CSO of Verogen. “We’re excited to partner with Hamilton to bring the forensic genomics community an automated solution for the ForenSeq DNA Signature Prep Kit. As we see wider adoption of the MiSeq FGx instrument, ForenSeq kit and software into forensic casework and databasing, the Hamilton solution automates up-front library preparation, making it possible to process more samples with less effort.”

The compact ID NIMBUS offers easy loading onto a high-density deck and includes four independent pipetting channels along with an ISO-certified positive pressure HEPA filtered clean air hood and UV filter to safeguard sample integrity. The software guides users through each step, and subsequent run files, including all robotic actions and sample locations, may be downloaded as part of case file documentation. Hamilton’s liquid handling tips feature patented CO-RE^® (Compressed O-Ring Expansion) technology; with a precise lock-and-key fit that creates an air-tight seal and facilitates pressure sensing used for liquid level detection without creating splatter/aerosolization upon tip ejection. The system offers 2D and 1D barcode scanning to eliminate transcription errors and enable complete tracking documentation that includes a chain-of-custody record for each sample, correlated with its case and assigned analyst.

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