Capto™ Blue Affinity Chromatography Medium Launched by GE Healthcare

GE Healthcare’s Capto Blue affinity chromatography medium is designed for the capture of human serum albumin (HSA), as well as purification of HSA fusion proteins, blood coagulation factors, enzymes, and recombinant proteins at laboratory and process scales. Developed from Blue Sepharose™ 6 Fast Flow, Capto Blue medium is more chemically stable and has a more rigid agarose base matrix than its predecessor. These improvements allow faster flow rates and larger sample volumes, leading to higher throughput and improved process economy.

Depending on the target molecule, the ligand functionality of Capto Blue may be modified through the use of appropriate buffer salts and buffer conductivity to increase selectivity. Capto Blue medium’s chemical stability also ensures tolerance for sodium hydroxide, enabling repeated cleaning-in-place (CIP) procedures to remove tightly bound impurities and maintain capacity.

Capto Blue medium is available as an easy-to use prepacked HiScreen™ column for fast small-scale purification and process development.