Complete Kit Assures Effective Hepatocyte Isolation

Worthington will showcase a new Hepatocyte Isolation System, developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. Visit booth #400 to learn more, and enter a draw to win a new Apple iPod.

To further minimize lot-to-lot variation, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells. Each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimized protocol for five separate adult rat liver perfusions. The method is based on that described by Berry, M.N., modified by Seglen, P.O., and further optimized in conjunction with several collaborating researchers.

At the booth, complete a short ʻPrimary Cell Isolationʼ survey, and request a copy of Worthington's new updated Tissue Dissociation Guide that is available to assist researchers with selecting enzymes, developing and optimizing protocols and better understanding the process of enzymatic primary cell isolation and tissue dissociation. The guide discusses the enzymes most commonly used, their handling and specificity, and includes hundreds of new detailed cell and species-specific references.

For more information, please visit the company article page.