Considerations for multiplex live cell imaging

Live cell imaging experiments are key to understanding dynamic processes. They allow us to visually record cells in their living state, without artifacts that could be introduced by fixation or termination of the various living processes. With live cell imaging, it is possible to observe dynamic processes in cells, tissues, or whole organisms in their natural environment, rather than just obtaining endpoint images and data. For example, you can study co-localization of cells or proteins to see if the different targets are positioned close to one another and observe how they interact. In this application eBook from Leica Microsystems, explore the use of multicolor, or multiplex, microscopy for live cell imaging, some key aspects of setting up a successful multicolor live cell experiment, and how to avoid some of the major pitfalls.