Cost-Effective Glutathione Agarose for GST-Tagged Protein Purification from Thermo Fisher Scientific

The new Thermo Scientific Pierce Glutathione Agarose family of products allows cost-effective purification of GST-tagged proteins from cellular lysates. Pierce Glutathione Agarose consists of glutathione linked to agarose beads though a 12-atom spacer which minimizes steric hindrance effects during purification.

The 6 percent beaded agarose and highly purified glutathione limit nonspecific binding and result in a high binding capacity of greater than 25mg of GST per milliliter of settled resin. The purification resin can be reused at least five times without loss in functionality.

The affinity purification of GST-tagged proteins using Pierce Glutathione Agarose is a simple, one-step process. Bound GST-tagged proteins are easily eluted with a buffer containing Thermo Scientific Reduced Glutathione. If the GST fusion protein contains a Factor Xa protease site, then the protein can be cleaved from the GST tag with Thermo Scientific Factor Xa.

Pierce Glutathione Agarose is available in numerous formats and as a standalone resin. Prepacked spin columns in a number of bed volumes are supplied alone or in kits. In addition, Pierce Glutathione Agarose comes in prepacked chromatography cartridges for FPLC applications.