Discovering cellular organelles with illumination microscopy

An organelle is a subcellular structure that contributes to a variety of cellular functions through its molecular composition and environmental interactions. CrestOptics discusses the significance of organelles in cellular functions and the limitations of conventional fluorescence microscopy in studying subcellular structures smaller than 200 nm. DeepSIM, a SIM super-resolution module that is compatible with any existing upright or inverted microscope with a camera port, is presented as a reliable, easy-to-use, and affordable solution to study sub-cellular structures with an XY resolution of 100 nm without requiring any special sample preparation protocol. DeepSIM's capability in visualizing various intracellular structures, such as endosomes, actin, mitochondria, tubulin, endoplasmic reticulum (ER), and lysosomes, at the subcellular level is also demonstrated.