Discussing the Advantages and Advancements of Selective Plane Illumination Microscopy for In Vivo Imaging

This application note relates information on SPIM, which has the potential to perform unperturbed in vivo imaging at cell-level resolution. SPIM is a fluorescence microscopy technique that uses a focused light-sheet to illuminate the specimen from the side. SPIM achieves excellent resolution at high penetration depths while being minimally invasive at the same time. SPIM offers a number of advantages over established techniques, such as strongly reduced photobleaching, high dynamic range, and high acquisition speed. These features make SPIM a popular technique for studying development.