Endonuclease treatment in downstream processing of virus-like particles

Removing host cell DNA is crucial in the production of bionanoparticles (BNPs) like viruses, viral vectors, and virus-like particles (VLPs). However, challenges are met in purification of VLPs as processes often produce low yield and low purity. Discover how ArcticZymes has formulated a treatment process combining flow-through chromatography with endonuclease cleaving to remove DNA and chromatin digestion products. Explore how the salt-active endonuclease M-SAN performs significantly better in removing DNA in the form of chromatin compared to benzonase, improving VLP yield and purity. Plus, by performing M-SAN treatment before flow-through chromatography, the capacity of heparin affinity chromatography is improved by at least five-fold.