Full-Length Proteins from Formalin-Fixed Tissue

As the standard method for histological tissue preparation, millions of Formalin-Fixed Paraffin-Embedded (FFPE) samples are classified and archived each year.

Now, the Qproteome FFPE Tissue Kit opens this vast, highly valuable resource up to proteomic and protein expression analysis by enabling efficient extraction of full-length proteins suitable for western blotting and protein arrays.

Because formalin fixation creates chemical crosslinks, stabilizing samples for histological analysis but rendering any subsequent protein analysis (e.g., by western blotting) impossible, analysis of FFPE tissue has been limited to histochemical techniques. The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse the crosslinking process and deliver full-length proteins ready for analysis by western blotting and protein array, with subsequent immunodetection. For the very first time, proteins or pathways of interest can be investigated in archived FFPE tissues.

The Qproteome FFPE Tissue Kit offers a unique tool for analysis of samples whose pathological status has been determined, but whose precise protein makeup has until now remained inaccessible. Such samples — for example, taken over a course of treatment or from different groups of probands — offer a rich source of information for comparative proteomics and biomarker studies.