High-throughput SPE method enables rapid and sensitive determination of a novel anxiolytic agent in human plasma

Porvair Sciences Ltd has released an application note that describes an automated SPE method for preparing samples for a rapid assay of a novel anxiolytic compound in human plasma, which employs liquid chromatography with tandem mass spectrometric (LC/MS/MS) detection.

Traditional methods employing Solid Phase Extraction (SPE) and LC/MS/MS were found not to have the throughput required to match the capacity of the assay that was being used to support elucidation of pharmacokinetic parameters of the target compound in Phase 1 trials. The SPE method was therefore adapted to employ the Microlute™ - a high performance 96-well SPE microplate system. The application note describes how the Microlute™ system enables a batch of samples to be prepared for injection in less than half the time that is required to analyse them.

The developed method using the Microlute™ was validated over a concentration range of 100 to 10,000 pg /ml. Recovery of the SPE method was approximately 60%, and consistent across the calibration range. The authors describe how the Microlute™ SPE method, unlike protein precipitation sample preparation, is free from LC/MS matrix effects that may influence the ionisation of the compound(s) of interest. The method meets all regulatory requirements for clinical studies and is suitable for the analysis of large numbers of samples.

Developed in conjunction with a leading pharmaceutical company the Microlute™ has become the benchmark for high throughput sample preparation across a range of applications. The innovative design of the Microlute™ from Porvair Sciences Ltd offers all the advantages of automated and high throughput SPE sample preparation in a convenient microplate format capable of rapidly processing 96 samples in one go repeatedly and precisely.

Constructed from a single piece of moulded high quality plastic means that Microlute™ will not bend or distort because individual SPE cartridges do not have to be repeatedly plugged in and out. Using a proprietary sorbent slurry loading technique, Porvair has eliminated the channelling effects often limiting the performance of dry powder loaded SPE columns. Each well on the Microlute™ has an individual drain spout ensuring 100% sample transfer and zero crossover contamination. Working closely with the leading automated liquid handling companies has ensured that the Microlute™ delivers high productivity, trouble-free operation with all robotic sample handling and preparation systems.

For copy of Microlute™ application note 2 please contact Porvair Sciences.