Hot Applications: 6 Phenotypic Assay Methods from BioTek

BioTek Instruments, Inc. offers six detailed methods to help drug discovery and life science researchers improve their phenotypic assays, which are available to download for free.

Phenotypic assays are replacing drug target-based assays in many early drug discovery processes, as there is no requirement to understand the drugs mechanism of action.

Automated digital fluorescence microscopy using microplates is a preferred method for phenotypic assays as multi-parametric data can be obtained using a wide array of fluorescent probes to quantify phenotype changes.

The Cytation Cell Imaging Multi-Mode Reader and the MultiFlo FX Multi-Mode Dispenser from BioTek are well equipped to automate phenotypic assay workflows. These six methods describe how they can perform common operations such as cell seeding into microplates, media exchanges, reagent additions and automated digital microscopy common to phenotypic assays.

Download them now for free:

Measuring Real-time Cellular Respiration and Mitochondrial Function using Fluorophoric Biosensors

Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging

Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy

Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity

Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate Imager

Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes