How to build a better qPCR assay

Processing published qPCR data to select the best experimental framework requires a significant amount of time, expertise and resources. LGC Biosearch Technologies, provides guidance on improving quantitative PCR (qPCR) assays by optimizing assay design and validation based on the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. LGC emphasizes considering factors like workflow, assay design optimization, primer and probe selection, assay validation, assay normalization, data analysis, and offers practical recommendations to enhance the accuracy and reliability of qPCR experiments.