How to design, optimize, and validate multiplex immunofluorescence panels

Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry (IHC) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra.

In this webinar, Michael J. Surace, Scientist II at AstraZeneca, will explore the biological and technical considerations when designing a panel, describe a linear process for developing, testing, and optimizing a panel, and provide an approach for technical validation of multiplex panels that detects and addresses known risks.

Key learning objectives:

• Learn about key factors to consider when designing a new mIF panel
• Explore an efficient process for developing and optimizing mIF panels using TSA-linked fluorophores and iterative staining
• Become familiar with the best use cases and advantages of most common mIF staining and imaging technologies
• Attain an adaptable scheme for testing and validating a novel mIF panel

The live webinar and Q&A session will take place on Thursday, March 26, at:

• 16:00 GMT
• 12:00 EDT
• 09:00 PDT
• 17:00 CET

Register to watch the full webinar here>>

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