How to optimize the focal height of your microplate reader measurements

In a microplate reader, the focal height is defined as the distance between the detection system and the microplate (i.e., the sample). The optimal focal height indicates the plane at which the microplate reader can detect the highest signal intensity of a sample. Therefore, the focal height setting is important for appropriately depicting all data points of your assay, and for reducing data variation. Explore how to optimize the focal height of your microplate reader measurements, and learn more about how performing focal height adjustments before your measurement ensures that your microplate reader will record your test samples correctly.