In-depth characterization of the major leukocyte cell subsets in human peripheral mononuclear cells

Flow cytometry is a powerful technique for in-depth analysis of different immune cell subpopulations of immune cells to gather a comprehensive assessment of the immune system. Spectral flow cytometry has led to advancements in our capabilities to support more markers, greater flexibility, and better data resolution. Agilent Technologies describes how a 45-color spectral flow cytometry panel was designed for the Agilent NovoCyte Opteon spectral flow cytometer to analyze the distribution of immune cells in a peripheral blood mononuclear cell (PBMC) sample.