Innovation: TF Chip Stem Cell kit- a new tool for stem cell characterization

The TF Chip Stem Cell kit enables profiling the status of a stem cell culture by 12 specific transcription factors in one assay. Two identical arrays are spotted on one slide and allow a direct comparison of two samples treated differently.

Double-stranded DNA containing the transcription factor specific binding sequence is spotted onto a coated glass surface. Mutated sequences serve as negative binding controls. The array is incubated with a nuclear protein extract and transcription factor proteins are monitored with antibodies specific for their activated status. Detection can be done with Silverquant^® technology or with fluorescence. This kit enables the analysis of the status of a stem cell culture in just one experiment in a convenient and simple workflow. It can be used for human, mouse or rat samples.