Leica DMI3000 B – the universal, inverted microscope for live cell imaging

Leica Microsystems presents the new generation Leica DMI3000 B inverted microscope, specifically designed for live cell research applications. The Leica DMI3000 B offers convenience and configuration possibilities that are unparalleled in this class of manual microscope. Leica’s new, integrated incident light fluorescence axis produces brilliant images for all fluorescence techniques. The Leica DMI3000 B also offers integrated modulation and phase contrast methods that do not require the use of special objectives.

The new Leica DMI3000 B is ideal for all manual fluorescence techniques. The system features a 5-position fluorescence turret for the fluorescence filter cubes. Leica’s Fluorescence Intensity Manager (FIM) regulates the illumination, as well as the aperture and field diaphragm and their centering. The fluorescence cubes feature light traps for background light reduction to produce a perfectly black background.

The Leica DMI3000 B offers a great variety of illumination options for transmitted light applications. The large selection of condensers has been supplemented by a special S40 condensor for micromanipulation with a working distance of 40mm, which is also suitable for Integrated Phase Contrast (IPH) and Integrated Modulation Contrast (IMC). Leica has improved its IMC to increase the depth of field, which increases the spatial visibility of thicker specimens. With the S40, Leica now offers five different condensers with different working distances (1, 23, 28, 40, and 70mm). Differential Interference Contrast (DIC) is also possible with the S70 condensor. For the budget-conscious user, a transmitted light arm is available with an integrated 30W lamp house.

Micromanipulation requires a meticulously aligned microscope system. For this purpose, Leica Microsystems offers a variety of micromanipulators (mechanical, electric, and hydraulic) that work seamlessly with the Leica DMI3000 B. A special stage for easy handling of the manipulators, and a variety of condensers with different working distances configure the DMI3000 B as a complete micromanipulation system.

Integrated Modulation Contrast (IMC) is ideal for non-colored, low-contrast specimens. Leica integrates the modulator outside of the objective, a method that is considerably easier to manage and more flexible in its application. Instead of special objectives, a variety of brightfield or phase objectives can be used. Via the freely accessible modulators, the image impression can now be optimized for contrast, resolution, depth of field, and relief impression to best view the specimen.

To create ideal climate conditions while examining live specimens, Leica offers a variety of incubators, temperature sensors, and heatable microscope stages. The Leica BL Incubator consists of a single climate chamber that fully integrates with all components, such as micromanipulators, scanning stages or cross-stages.