Life Technologies Assists in a Major Breakthrough in Transient Gene Expression

Evitria SA has succeeded in obtaining titers exceeding 1 gram per liter in transient through using Life Technologies’ Invitrogen FreeStyle™ CHO-S Cells. Evitria’s rapid protein expression platform now allows companies to perform their in vitro and in vivo studies with protein transiently produced in the same host system as used later for production of stable cell lines.

Comments Dr. Sebastien Chenuet, Founder and Principal Investigator at evitria: “Today, CHO cells are the preferred host system for stable cell lines. Yet, many companies perform their initial in vitro and in vivo studies with protein transiently produced in HEK293 cells. We see the reason for that in the challenge to produce sufficient amounts of protein transiently in CHO cells. Going forward, evitria’s rapid protein expression platform will enable companies to perform their preclinical and clinical studies with the same host system. This is especially crucial in light of the extensive literature that stresses the importance of glycosylation on protein functionality.”

Comments Dr. Markus Hildinger, Founder and Principal Investigator at evitria: “We did it again! Building on my inventions from my sabbatical year at the École Polytechnique Fédérale de Lausanne (EPFL), we were able to extend 1 gram per liter titers in transient gene expression beyond HEK293 to CHO cells. This accomplishment positions evitria amongst the leading companies in the field of rapid protein expression and allows us to offer our customers milligram to gram quantities of protein at highly competitive prices starting as low as USD 300. Of course, we are also open to license evitria’s rapid protein expression platform to interested third parties.”