LSM 5 MP Laser Scanning Microscope from Carl Zeiss – The Specialist for Multiphoton Microscopy

With the LSM 5 MP system, Carl Zeiss MicroImaging GmbH is introducing a laser scanning microscope specially configured for multiphoton microscopy that offers an excellent price/performance ratio.

In this way, the company is reacting to the increasing importance of this method for examinations on living biological tissue, allowing them to be performed as gently as possible.

Highly efficient coatings on the optics ensure minimal power losses of the directly coupled femtosecond lasers. Leading edge filter technology guarantees efficient suppression of the exciting light with simultaneous highly sensitive detection. Objective lenses adapted to the needs of multiphoton microscopy and featuring optical correction in the near infrared spectral range allow the optimum use of different IR excitiation wavelengths, also into ranges of over 1000 nm. As these wavelengths enable even greater depths of tissue penetration thanks to lower absorption and scatter, their use in multiphoton microscopy is particularly beneficial.

New standards are set by the W Plan-Apochromat objective lens with 20x magnification at a numerical aperture of 1.0 and a working distance of 2 mm. This objective lens is especially suitable for electrophysiology combined with multiphoton microscopy. It allows the imaging of larger areas of the specimen with excellent resolution and light collecting efficiency. With the LSM 5 MP and its adapted optics, Carl Zeiss MicroImaging is expanding its line of multiphoton systems which also includes the LSM 510 NLO and LSM 510 META NLO.