Most Downloaded Clinical Chemistry Application Articles

Application articles are uploaded daily to the SelectScience article library

We have compiled a list of the most downloaded clinical chemistry application articles this year so far. The list includes a number of mass spectrometry application notes, as well as the poster abstracts from the recent Binding Site International Symposium on free/heavy light chain analysis.

1. Hematology Reports: 7th International Symposium on Clinical Applications of Free Light Chain and Heavy/Light Chain Analysis

Read abstracts from all posters submitted to The Binding Site’s 7th International Symposium on Clinical Applications of Free Light Chain and Heavy/Light Chain Analysis. Posters cover a range of topics, including: Monitoring Monoclonal Gammopathies; Diagnostic Algorithms for Multiple Myeloma; Risk Stratification of Monoclonal Gammopathies; Case Reports; AL Amyloidosis; Method Evaluation and Comparison; Monoclonal Free Light Chain and Renal Implications; Other Uses of Free Light Chain and Heavy Light Chain; Free Light Chains in Multiple Sclerosis; and Polyclonal Free Light Chains.

2.A Sensitive, Specific, Accurate, and Fast LC/MS/MS Method for Measurement of Nicotine, Cotinine, 3-Hydroxycotinine, Nornicotine and Anabasine in Human Urine

This application note illustrates a method simultaneous analysis of nicotine, two of its major metabolites, cotinine and 3-hydroxycotinine, as well as nornicotine and anabasine from human urine samples. The method described uses Strata™-X-C solid phase extraction (SPE) cartridges for sample clean-up and concentration, followed by fast (<6 min) LC/MS/MS analysis using a Gemini® NX-C18 column.

3. Extractables do not Affect Downstream Vitamin D Analysis by LC-MS/MS when Using the Tecan AC Extraction Plate™

Quantification of small molecules in biological samples (such as serum, plasma, urine or blood) by LC-MS is generally only performed after a sample clean-up step. This is due to the presence of specific matrix constituents – such as proteins and phospholipids – which might negatively affect the analysis. The Tecan AC Extraction Plate simplifies sample preparation by efficiently removing most matrix components – such as proteins and salts – without any precipitation, filtration, centrifugation and evaporation steps.

4. Automated, High-Throughput LC-MS/MS Workflow for the Analysis of 25-Hydroxyvitamin D2/3 and 3-Epi-25-Hydroxyvitamin D3

In this application note, an automated, high-throughput LC-MS/MS workflow was developed for the analysis of 25-hydroxyvitamin D2/3 and 3-epi-25-hydroxyvitamin D3 in human serum for research laboratories.

5. Automation of a Method for the Analysis of a Pain Panel by LC-MS/MS

In the work presented here, an LC-MS/MS method for the analysis of a pain panel comprising 15 analytes has been developed and automated from start to finish, using the AB SCIEX 3200 QTRAP® LC/MS/MS System for analysis. Not only does method automation eliminate human error, thus increasing reproducibility, it also eliminates subjectivity during data processing and furthermore has the potential to save a great deal of time.

6. Validation of Automated Liquid-Liquid Extraction of 25-Hydroxy Vitamin D from Whole Blood or Spiked BioCell Material Samples Using VERSA Workstation

Measurement of circulating levels of 25-hydroxy vitamin D is important in the diagnosis of intestinal mal-absorption and vitamin D deficiency or intoxication in humans. This has led to exponential growth in clinical diagnosis tests for 25-hydroxyvitamin D in blood serum. Some laboratories have developed assays using LC-MS/MS tandem mass spectrometry or HPLC. However, sample preparation to meet the requirements of these innovative methods is a cumbersome process if performed manually. To assist the sample preparation, this application note presents the VERSA Workstation for automated liquid-liquid extraction of 25-hydroxyvitamin D from blood samples.