Navigate the Fluorescent Minefield with Olympus

23 May 2007

Olympus makes it simple for researchers using fluorescent microscopy to correctly match dyes to filter-sets by producing a poster on the Spectra of Fluorescent Dyes. The poster enables researchers to see at a glance the perfect dye and filter combinations so that they can get the best out of their systems. Copies of the ‘Spectra of Fluorescent Dyes’ poster can be obtained by contacting Olympus.

With so many different fluorescent dyes now available, it can be difficult and daunting to find the correct ones for an experiment. Some of the many considerations include: knowing the absorption/emission spectrum of the dye; finding two or more dyes with non-overlapping spectra so that multi-label studies can be performed; and determining the filter-sets that should be used. Olympus’s ‘Spectra of Fluorescent Dyes’ poster acts as an essential reference to help ‘map’ these dyes and filter-sets and aid your navigation of the “fluorescent minefield”.

Selected Absorption and emission spectra of all the major dyes - Alexa, Cyanine, Fluorescent Proteins, Xanthene, Indole - are covered and the 52 cm x 75 cm poster is ideal for walls and is large enough for the data to be easily seen. Copies of the ‘Spectra of Fluorescent Dyes’ poster can be obtained by contacting your local Olympus representative or through the website.

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