New Clinical PCR Master Mix Developed for Challenging Genotyping, CNV Experiments

Thermo Fisher Scientific continues to create products that meet the changing needs of clinical real-time PCR (qPCR) customers by launching Applied Biosystems TaqPath ProAmp PCR Master Mix.

Applied Biosystems™ TaqPath™ ProAmp™ Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation (CNV) protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mixes are designed to deliver sensitive and reproducible results from genomic DNA targets.

Two formulations of this Applied Biosystems multiplex assay-compatible master mix are available: TaqPath ProAmp Multiplex Master Mix with Mustang Purple for multiplexing up to four targets; and TaqPath ProAmp Master Mix, which includes ROX™ as the passive reference dye. Both mixes enable measurement of FAM™, VIC™, and ABY™ dye or other dyes with similar emission wavelengths. The multiplex version also allows the use of JUN™ dye, or other dyes with similar emission wavelengths, in the channel typically used to measure ROX dye for single nucleotide polymorphism (SNP) genotyping and other higher multiplex applications.

These two formulations join the line of Applied Biosystems TaqPath General Purpose Reagents—qPCR and RTqPCR—master mixes manufactured in an ISO 13485 certified facility to help ensure manufacturing excellent manufacturing consistency. 1Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.

Features:

• High data quality--high specificity, dynamic range1, and reproducibility for genotyping and copy number determination
• Tolerance to inhibitors--compatible with samples prepared from human or animal sources (buccal swabs, blood, and card punches)
• 72-hour pre-PCR benchtop stability--proprietary Dual-Lock™ Taq DNA Polymerase hot-start mechanism for setup on automated workflows
• Multiplexing breadth—Detect up to 4 targets per reaction
• Manufacturing consistency—manufactured in an ISO 13485-certified facility