New Eppendorf Eporator® for Safe, Highly Efficient Transformation

The newly launched Eppendorf Eporator® offers a fast, simple and safe way to transform bacteria, yeast and other microorganisms with DNA / RNA. Results are highly reproducible and compared with chemical methods, electroporation yields significantly higher transformation efficiency.

Exposing bacteria or yeast strains to short, high voltage electrical pulses enables macromolecules, such as plasmid DNA, to diffuse into the cell through temporary pores in the cell membrane. Designed to deliver ideal conditions for electroporation of bacteria and yeast, the Eppendorf Eporator® has been shown to give transformation efficiencies ten times higher than with chemical transformation (heat shock method)¹, and 50 per cent higher than the earlier Eppendorf Electroporator 2510².

The transformation of bacteria in order to amplify recombinant DNA is often carried out using chemical transformation. Although reliable and cost-effective, this method is very time-consuming and the transformation efficiency can be too low. The new Eppendorf Eporator® not only saves valuable time and delivers ten times higher transformation rates, crucially the instrument is extremely user friendly. Two new program buttons allow storage and recall of most commonly used parameters and simple one-button operation ensures intuitive use for faster sample handling.
Eppendorf Eporator® has a compact, space-saving design for easy storage and transport and comes with a USB port facilitating export of data for analysis and GLP-compliant documentation.

This new electroporator incorporates special safety features which maximise user protection. Safe electronics and an integrated electroporation chamber eliminate voltage leaks. Eppendorf electroporation cuvettes are available in three sizes and with different gap widths. Application protocols for the electroporation of various bacteria and yeast strains can be found at the Company article page link top right of this page.

¹ Eppendorf Application Note 216, September 2009
² Eppendorf Application Note 219, October 2009