New Gel System for High Quality Protein Electrophoresis

GE Healthcare’s horizontal Amersham ECL Gel System is designed for high quality protein electrophoresis enabling efficient, high-quality and reproducible protein separation. Offering high sensitivity and resolution, it works with standard protein detection protocols and is easy to integrate into the Amersham ECL Western blotting workflow.

The core of the system is Amersham ECL Gel, a precast gel available in a variety of concentrations and well configurations. Gels are processed using Amersham ECL Gel Box, a dedicated electrophoresis unit with horizontal design, and significantly increased ease of use compared to vertical systems.

Everything required to access and cut the gel is included in the cassette. At 1.4 mm thickness, it is thicker than many other precast and hand-cast gels, providing peace-of-mind when handling and reducing the risk of gel breakage in downstream processing.

The Amersham ECL Gel System aims to make PAGE as easy as gel DNA electrophoresis. The horizontal format allows for easier sample application, gives a full view of the entire electrophoresis run and requires virtually no assembly, reducing preparation time. The gel contains no SDS, making it appropriate for protein analysis under native and denaturing conditions, depending on the choice of running and sample loading buffers. Coupled with lower buffer consumption and no risk of leakage between buffer chambers, the gel system allows the resolution of complex samples.

Amersham ECL Gel is compatible with standard Laemmli buffers and has a shelf life of up to 12-months, without any compromise in performance. For added convenience, a 10x concentrated running buffer for SDS-PAGE is available, as well as pre-cut Hybond™ membranes for Western blotting applications.