New HPLC Columns Enhance Peak Capacity

Thermo Fisher Scientific Inc. has expanded its portfolio of Thermo Scientific Accucore columns with a range of columns for biomolecule separations. Available in analytical, micro and nano formats, the new AccucoreT^M 150-C18 and Accucore 150-C4 columns are designed to improve protein and peptide assay robustness by enhancing peak capacity and run times at moderate operating pressures.

Thermo Fisher introduced the new Accucore HPLC and nanoLC columns at HPLC 2012 in Anaheim, Calif. within Thermo Scientific booth 121.

The 150 angstrom pore diameter of the solid core particles is designed to provide the optimal combination of retention time and resolution for peptides and proteins. The Accucore 150-C18 has been further optimized for biomolecules and protein digests through bonding of C18 ligands onto the porous outer layer of the particles. The low hydrophobicity C4 ligand used in the Accucore 150-C4 column enables fast protein separations.

“In the pharmaceutical industry, biological drugs continue to make gains compared to conventional drugs, with a significant rise anticipated between 2010 to 2016*,” said Darren Thomas, business director, Chromatography Consumables Solutions, Thermo Fisher. “We believe that the Accucore family of columns will accelerate time to market for these large-molecule based drugs.”

Accucore columns are based on Core Enhanced Technology^TM, featuring solid core particles engineered to an average size distribution of 1.12. The result is high-resolution separations with backpressures significantly lower than those of UHPLC. Advanced bonding technology produces excellent peak shapes through high bonding coverage and minimized secondary interactions. The automated packing processes produce uniform, tightly-packed columns that are robust and reproducible.