New Vitamin-Rich Peptone from Oxoid Improves Microbial Growth
19 Apr 2007Oxoid, a world leader in microbiology, has extended its range of Laboratory Preparations to include Tryptone X (product code LP0642), a new vitamin-rich pancreatic digest of casein. Tryptone X is obtained using an advanced process that allows the natural release of vitamins into the product. As a result, this highly nutritious peptone encourages improved growth rates for a variety of important micro-organisms.
Tryptone is an excellent source of amino nitrogen and is often used as a constituent in microbial culture media. Its high tryptophan content makes it ideal for use in media that test for the indole reaction. It can also be used to detect ‘flat-sour’ or ‘sulphide’ spoilage organisms in the canning industry and in sterility testing media1.
In addition to complying with the specification for a pancreatic digest of casein in the U. S. Pharmacopoeia2, Tryptone X also provides an excellent source of vitamins. It may be used as a constituent of media used in fermentation processes to produce antibiotics, extra-cellular protein, vaccines, interferon and diphtheria toxoid. It could also be used in industrial biotechnology for the production of recombinant proteins and speciality chemicals (ethanol, mannitol, bioplastic PHA, heavy duty detergents), in the production of monoclonal antibodies and for research purposes.
In growth studies, Tryptone X demonstrated superior performance compared to a standard Tryptone product in the growth of E. coli (ATCC® 25922™), Lactobacillus brevis (ATCC® 8291™), Staphylococcus aureus (ATCC® 9144™), Citrobacter freundii (ATCC® 8090™) and Enterobacter aerogenes (ATCC® 13048™)3. In addition, both Oxoid Tryptone X and Oxoid Tryptone demonstrated improved growth curves for Bacillus subtilis (ATCC® 6633™) compared to competitor Tryptone products3. Furthermore, Tryptone X offers excellent uniformity, with proven lot-to-lot consistency3.
Together with Oxoid Tryptone (product code LP0042) and Oxoid Tryptone T (product code LP0043) (used in the production of Clostridium tetani), the addition of Tryptone X allows Oxoid to offer an excellent selection of products for formulations that require a pancreatic or tryptic digest of casein.
References:
1European Pharmacopoeia, 5th edition, 2005.
2United States Pharmacopoeia USP30-NF25, 2007.
3Information on file, Oxoid Limited.
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