Preparation of Peptide N-Glycosidase F Digests for HPAE-PAD Analysis

This application note describes a simple sample pretreatment method for small-scale PNGase F digests that use protein denaturation. After pretreatment, the samples are amenable to HPAE-PAD oligosaccharide analysis with the CarboPac PA200 column, making it possible to study and monitor the oligosaccharide content of a glycoprotein.