Promega Trypsin/Lys-C Mix Enhances Proteolysis of Difficult-to-Digest Proteins

Promega Corporation announces the launch of Trypsin/Lys-C Mix, Mass Spectrometry Grade. A unique combination of Trypsin Gold and rLys-C proteases, the new mix increases peptide generation with in-solution protein digestion and works with even difficult to digest proteins.

Enhanced proteolytic activity achieved through use of Trypsin/Lys-C Mix can result in a 20-40% increase in the number of peptides generated, enabling a higher number of proteins to be identified. Trypsin/Lys-C Mix is also tolerant to Trypsin-inhibiting contaminants, enabling researchers to generate mass spectrometry data from poor-quality sample material such as FFPE sections.

Many proteins are resistant to Trypsin due to tight conformation, and although protein denaturation enables proteolysis, Trypsin is inactive under these conditions. Promega’s Trypsin/Lys-C Mix overcomes this barrier as it remains active even under strong denaturing conditions, enabling digestion of even proteolytically resistant proteins.