Protein aggregation identified through UV-visible absorption spectroscopy

Misfolded or denatured proteins can associate in solution, forming insoluble aggregates. This process is often irreversible, effectively removing useful proteins from solution and making the detection of aggregates critical for further downstream use of protein solutions. This is particularly important when studying unstable or abnormal proteins, which are more likely to form aggregates. In this application note from Thermo Fisher Scientific, explore an alternative method for the detection of protein aggregates using UV-visible (UV-Vis) absorption spectroscopy, a technique that measures a sample’s light absorption.