Randox Announces a Highly Accurate Direct Clearance Method for HDL Cholesterol

HDL cholesterol is an important risk factor for cardiovascular disease. Accurate and precise measurements are therefore necessary to enable effective diagnosis and treatment of lipid disorders. The direct clearance method from Randox, is designed to enable accurate diagnosis and risk assessment even with abnormal samples.

There are several methods available for the measurement of HDL Cholesterol, the ultracentrifugation reference method although highly specific, is time consuming and difficult to perform involving lengthly sample preparation and analytical steps. Due to high costs and time constraints the use of this method is limited with routine laboratories preferring to use direct methods of measurement.

There are currently several direct methods available offering many advantages over traditional methods as they do not require sample pre-treatment and enable rapid analysis of HDL on most automated analysers. Despite performing well with normal samples many of these direct methods show reduced specificity and accuracy in samples containing abnormal lipoproteins e.g. patients with elevated levels of triglycerides or liver damage.

To overcome such issues with abnormal lipoproteins Randox has developed a clearance method for HDL cholesterol which offers all the advantages of a direct method without compromising performance in discrepant samples. The Randox direct clearance method is highly specific for HDL and works by completely removing all non-HDL components including abnormal lipoproteins in the first step of the reaction. This enhances the specificity of the assay and results in a high degree of accuracy even with samples containing high levels of triglycerides or bilirubin.

In addition to highly accurate results the Randox direct clearance method shows excellent agreement to the CDC ultracentrifugation reference method with a correlation coefficient of 0.99. Further benefits include the availability of fully automated applications for a wide range of clinical analysers; excellent precision compared to precipitation methods, excellent onboard stability and reduced labour, time and consumable costs. Furthermore, all reagents are liquid ready to use eliminating the risk of reconstitution errors, potential contamination and bottle to bottle variations.


Randox can also provide high quality reagents covering the full lipid profile including;
LDL Cholesterol
sLDL Cholesterol
Total Cholesterol
Triglycerides
Lipoprotein (a)
Apolipoprotein A-1, A-II, B, C-II, C-III and E

For more information please see the company article page.