Replicating water and fat soluble vitamins analysis on a modern HPLC system

With the increasing popularity of vitamin supplements, there is a need to ensure the products meet the content descriptions. High-performance liquid chromatography (HPLC) is an essential analytical tool for measuring supplements, ensuring products meet label claims. However, the wide range of chemical characteristics makes analysis of vitamins with a single mode of chromatography challenging. For highly non-polar, fat-soluble vitamins, analysis by normal phase is a preferred mode of chromatography, while water-soluble vitamins are often analyzed by hydrophilic interaction chromatography (HILIC) to address the retention of highly polar vitamins.

In regulated labs, the ability to migrate these methods to different systems can be essential as systems are updated and replaced. Therefore, having a single system that can perform both types of analyses with minimal changes is crucial. This poster from Waters Corporation shows how a HILIC method for water-soluble vitamins and a normal phase method for fat-soluble vitamins were successfully migrated from the Alliance™ 2695 System to the Alliance™ iS HPLC System. The Alliance iS HPLC System was configured for normal phase chromatography using check valves and outlet tubing.