Roche Launches New Tools for RNA Isolation and cDNA Pre-Amplification From FFPET Samples

Roche has announced the global launch of its new High Pure FFPET RNA Isolation Kit as well as the RealTime ready cDNA Pre-Amp Master & Primer Pools. Formalin-fixed, paraffin-embedded tissue (FFPET) samples from biopsies or micro-dissections are frequently used for retrospective studies, primarily in oncology. They also represent an increasingly important resource for biomarker research and investigating disease progression or drug responsiveness.

The new High Pure FFPET RNA Isolation Kit delivers RNA of excellent integrity and yield – two key factors in ensuring high-quality results in the diversity of downstream applications such as qRT-PCR, microarray analysis and sequencing. For comprehensive gene expression studies, the isolated RNA can be transcribed into cDNA and subsequently pre-amplified with the new RealTime ready cDNA Pre-Amp Master. This kit makes it possible to pre-amplify minute amounts of cDNA with customized primer pools that correspond perfectly to the primers which can be applied in subsequent qPCR analysis. This approach ensures unbiased amplification and a flexible fit to any customer’s genes of interest.

“Our new products provide highly convenient and flexible solutions to researchers who are analyzing gene expression in FFPET samples, while ensuring excellent result quality,” said Gerd Haberhausen, Business Head of qPCR and Sample Preparation Systems. “By offering customized, aligned primer sets alongside our new RNA isolation kit, we are able to provide a complete and reliable workflow to scientists investigating the mRNA expression profiles of complex biological pathways.”

The High Pure FFPET RNA Isolation Kit’s superb performance has been proven by analyzing FFPET samples of different ages and by demonstrating its applicability in biomarker research. It produces powerful signals downstream in qPCR, microarray analysis, and sequencing. RNA from formalin-fixed, paraffin-embedded tissue, macro-dissected and laser-micro-dissected samples, and biopsies can be isolated in just 2.5 hours, including deparaffinization.

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