The Easy Way to Remove Protein from your Samples

The new QPF 96-well Protein Precipitation Filter plates, from Anachem, now offer an easier method for elimination of protein from biological fluids.

They provide unrivalled reliability, whether used manually or as part of an automated system. The special multi-well filtration microplates simplify and speed up sample preparation compared to traditional methods such as centrifugation.

QPF plates efficiently reduce liquid handling in applications such as quantitative drug analysis by LCMS/MS. They simplify automation, saving technician time and valuable resources. Acetonitrile is added to the plate wells, followed by sample. After a two minute incubation vacuum or positive pressure is applied, the resulting filtrate is free of precipitated protein.

Anachem QPF plates are constructed using an innovative Double Filter System, consisting of a top filter (100-150ìm) to remove large particulates and a bottom filter (10ìm) to eliminate any small particles. By allowing the larger particles to precipitate the plate’s top layer remains unblocked so that the bottom layer can continuously remove particles, even with high particulate loads. Each filter has the ability to hold both plasma and organic solvents without leakage, enabling simple protein precipitation to be employed.

Anachem’s extensive quality control procedures ensure the highest quality product by using advanced testing equipment and performing rigorous quality inspections. Each new batch of plates are tested on a Gilson SPE automated system to maintain reliability and eliminate batch-to-batch variation. QPF plates are suitable for use with manual protocols or automated systems using robotic equipment such as the Gilson SPE 215 simultaneously processing 8 samples) or the 925 Workstation which simultaneously processes 96 samples.